{"id":4175,"date":"2022-12-20T18:04:37","date_gmt":"2022-12-20T18:04:37","guid":{"rendered":"https:\/\/genetica.incor.usp.br\/?page_id=4175"},"modified":"2023-02-10T18:56:53","modified_gmt":"2023-02-10T18:56:53","slug":"cell-culture","status":"publish","type":"page","link":"https:\/\/genetica.incor.usp.br\/?page_id=4175","title":{"rendered":"Cell Culture &#038; IPS"},"content":{"rendered":"\r\n<div class=\"card\">\r\n    <div class=\"card-body\">\r\n        <div class=\"container\">\r\n            <h2 style=\"margin-bottom: 20px;\">Cell Culture &#038; IPS<\/h2>\r\n            <div class=\"pbs-row\" data-width=\"full-width-retain-content\" style=\"min-height: 200px; position: relative; margin: -50px 0px 47px;\">\r\n                <div class=\"pbs-col\" style=\"justify-content: center; flex-basis: 48.6805%; padding: 50px 11px 0px 10px;\">\r\n  \r\n                <\/div>\r\n                <div class=\"pbs-col\" style=\"justify-content: center; flex-basis: 51.3195%; padding: 40px 11px 0px 10px;\">\r\n                <p style=\"margin-top: 5px;\"><span style=\"font-size: 12pt;\">The cell and tissue physiology rooms are the area inside the Laboratory of Genetics and Molecular Cardiology dedicated exclusively to cell and tissue cultivation.<\/span><\/p>\r\n                <p style=\"margin-top: 5px;\"><span style=\"font-size: 12pt;\">In LCGM there are two types of cell culture: one for the cultivation of human and animal cells and the other, with more strict environment control, for the cultivation of Human Induced Pluripotent Stem Cells (hiPSCs) and tissue culture (explant).<\/span><\/p>\r\n                <p style=\"margin-top: 5px;\"><span style=\"font-size: 12pt;\">Specifically, in this last room there is equipment that simulates changes in O2 concentration, mechanical forces and electrical stimuli found in blood vessels and in the heart. Both rooms are equipped with laminar flow hoods, incubators, water baths, benchtop centrifuges, phase and fluorescence microscopes.<\/span><\/p>      \r\n                <p style=\"margin-top: 5px;\"><span style=\"font-size: 12pt;\">They have a restricted access environment with asepsis and temperature control, in order to avoid contamination by microorganisms.<\/span><\/p>\r\n            <\/div>\r\n            <\/div>\r\n            <div>\r\n                <h2 align=\"center\" class=\"itensLinha\">Activities<\/h2>\r\n                <div class=\"row centro\" style=\"display: flex; justify-content: center; align-items: center;\">\r\n                    <button type=\"button\" class=\"btn btn-primary ajustesTopics\"><i class=\"far fa-bookmark\"><\/i> Molecular and functional biology <\/button>\r\n                    <button type=\"button\" class=\"btn btn-primary ajustesTopics\"><i class=\"far fa-bookmark\"><\/i> Gene editing <\/button>\r\n                    <button type=\"button\" class=\"btn btn-primary ajustesTopics\"><i class=\"far fa-bookmark\"><\/i> Cell therapy <\/button>\r\n                    <button type=\"button\" class=\"btn btn-primary ajustesTopics\"><i class=\"far fa-bookmark\"><\/i> Tissue culture (explant) <\/button>\r\n                    <button type=\"button\" class=\"btn btn-primary ajustesTopics\"><i class=\"far fa-bookmark\"><\/i> 3D cell culture <\/button>\r\n                    <button type=\"button\" class=\"btn btn-primary ajustesTopics\"><i class=\"far fa-bookmark\"><\/i> Disease modeling <\/button>\r\n                <\/div>\r\n            <\/div>\r\n            <\/br>\r\n        <\/div>\r\n    <\/div>\r\n<\/div>\r\n<br>\r\n<br>\r\n<br>","protected":false},"excerpt":{"rendered":"<p>Cell Culture &#038; IPS The cell and tissue physiology rooms are the area inside the Laboratory of Genetics and Molecular Cardiology dedicated exclusively to cell and tissue cultivation. In LCGM &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/genetica.incor.usp.br\/?page_id=4175\" class=\"more-link\">Read more<span class=\"screen-reader-text\"> &#8220;Cell Culture &#038; IPS&#8221;<\/span><\/a><\/p>\n","protected":false},"author":438,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4175","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=\/wp\/v2\/pages\/4175","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=\/wp\/v2\/users\/438"}],"replies":[{"embeddable":true,"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4175"}],"version-history":[{"count":9,"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=\/wp\/v2\/pages\/4175\/revisions"}],"predecessor-version":[{"id":4357,"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=\/wp\/v2\/pages\/4175\/revisions\/4357"}],"wp:attachment":[{"href":"https:\/\/genetica.incor.usp.br\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4175"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}